Journal: Glia

Article Title: Targeting Connexin 43 in Retinal Astrocytes Promotes Neuronal Survival in Glaucomatous Injury.

doi: 10.1002/glia.70013

Figure Lengend Snippet: FIGURE 1 | Microbead occlusion model of glaucoma induces RGC loss. (A) Time points of experimental analysis. (B) Illustration of intracameral injection of polystyrene microbeads into WT and Cx43KO mice. (C) Data show sustained elevation of IOP (p < 0.001) in WT (n = 58) and KO (n = 50) compared to PBS-injected control eyes for 8 weeks after initial microbead injections at day 0. The second injection was administered 4 weeks after the first injection (arrow). There is no significant difference in IOP elevation between WT and KO mice (p = 0.87, Student's t test). (D) Immunofluorescent images of Brn3a positive RGCs in wholemount retinas of control (left; non-treated; NT) and bead-injected retinas (right) of WT mice at 8 weeks af- ter microbead injection. (E) Quantification of Brn3a positive cells in control (n = 18) and 4 (n = 8) and 8 (n = 10) weeks after glaucomatous injury. ***p < 0.001, Student's t test. Scale bar 50 μm.

Article Snippet: The following primary antibodies were used: rabbit anti- Cx43 (Millipore Sigma, # C6219, 1:1000), goat anti- Brn3a (Santa- Cruz, # sc- 31,984,1:500), mouse anti- Brn3a (Santa- Cruz, # sc- 8429, 1:100) and guinea pig antiRBPMS (PhosphoSolutions, # 1832, 1:500) for staining of RGCs; mouse anti- GFAP (Millipore Sigma, MAB 360, 1:1000) and rabbit anti- Sox9 (Sigma- Aldrich, # AB5535, 1:1000) for staining of astrocytes; goat anti- Iba1 (Abcam, # AB5076, 1:500) and rat anti- CD68 (Biolegend, # 137001, 1:500) for labeling of microglia (Table 1).

Techniques: Injection, Control

Journal: Glia

Article Title: Targeting Connexin 43 in Retinal Astrocytes Promotes Neuronal Survival in Glaucomatous Injury.

doi: 10.1002/glia.70013

Figure Lengend Snippet: FIGURE 10 | Cx43 deletion is neuroprotective in the optic nerve crush model. (A) Representative images of nontreated control (left) and ONC (right) retinas of WT mice. (B) Representative images of nontreated control (left) and ONC (right) retinas of Cx43KO mice. (C) Quantification of Brn3a + RGCs after ONC in WT and Cx43KO mice. Deletion of Cx43 increases RGC survival by 2-fold. ns, not significant, ***p < 0.001, ****p < 0.0001, Student's t test. Scale bar 50 μm.

Article Snippet: The following primary antibodies were used: rabbit anti- Cx43 (Millipore Sigma, # C6219, 1:1000), goat anti- Brn3a (Santa- Cruz, # sc- 31,984,1:500), mouse anti- Brn3a (Santa- Cruz, # sc- 8429, 1:100) and guinea pig antiRBPMS (PhosphoSolutions, # 1832, 1:500) for staining of RGCs; mouse anti- GFAP (Millipore Sigma, MAB 360, 1:1000) and rabbit anti- Sox9 (Sigma- Aldrich, # AB5535, 1:1000) for staining of astrocytes; goat anti- Iba1 (Abcam, # AB5076, 1:500) and rat anti- CD68 (Biolegend, # 137001, 1:500) for labeling of microglia (Table 1).

Techniques: Control

Journal: Glia

Article Title: Targeting Connexin 43 in Retinal Astrocytes Promotes Neuronal Survival in Glaucomatous Injury.

doi: 10.1002/glia.70013

Figure Lengend Snippet: FIGURE 1 | Microbead occlusion model of glaucoma induces RGC loss. (A) Time points of experimental analysis. (B) Illustration of intracameral injection of polystyrene microbeads into WT and Cx43KO mice. (C) Data show sustained elevation of IOP (p < 0.001) in WT (n = 58) and KO (n = 50) compared to PBS-injected control eyes for 8 weeks after initial microbead injections at day 0. The second injection was administered 4 weeks after the first injection (arrow). There is no significant difference in IOP elevation between WT and KO mice (p = 0.87, Student's t test). (D) Immunofluorescent images of Brn3a positive RGCs in wholemount retinas of control (left; non-treated; NT) and bead-injected retinas (right) of WT mice at 8 weeks af- ter microbead injection. (E) Quantification of Brn3a positive cells in control (n = 18) and 4 (n = 8) and 8 (n = 10) weeks after glaucomatous injury. ***p < 0.001, Student's t test. Scale bar 50 μm.

Article Snippet: # Primary antibody Host Company Catalog# Dilution 1 Cx43 Rabbit Millipore Sigma C6219 1:1000 2 Brn3a Goat Santa- Cruz sc- 31,984 1:500 3 Brn3a Mouse Santa- Cruz sc- 8429 1:100 4 RBPMS Guinea pig PhosphoSolutions 1832 1:500 5 GFAP Mouse Millipore Sigma MAB360 1:1000 6 Sox9 Rabbit Sigma- Aldrich AB5535 1:1000 7 Iba1 Goat Abcam AB5076 1:500 8 CD68 Rat Biolegend 137,001 1:500 10981136, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/glia.70013 by IN A SP - N E PA L , W iley O nline L ibrary on [07/04/2025].

Techniques: Injection, Control

Journal: Glia

Article Title: Targeting Connexin 43 in Retinal Astrocytes Promotes Neuronal Survival in Glaucomatous Injury.

doi: 10.1002/glia.70013

Figure Lengend Snippet: FIGURE 10 | Cx43 deletion is neuroprotective in the optic nerve crush model. (A) Representative images of nontreated control (left) and ONC (right) retinas of WT mice. (B) Representative images of nontreated control (left) and ONC (right) retinas of Cx43KO mice. (C) Quantification of Brn3a + RGCs after ONC in WT and Cx43KO mice. Deletion of Cx43 increases RGC survival by 2-fold. ns, not significant, ***p < 0.001, ****p < 0.0001, Student's t test. Scale bar 50 μm.

Article Snippet: # Primary antibody Host Company Catalog# Dilution 1 Cx43 Rabbit Millipore Sigma C6219 1:1000 2 Brn3a Goat Santa- Cruz sc- 31,984 1:500 3 Brn3a Mouse Santa- Cruz sc- 8429 1:100 4 RBPMS Guinea pig PhosphoSolutions 1832 1:500 5 GFAP Mouse Millipore Sigma MAB360 1:1000 6 Sox9 Rabbit Sigma- Aldrich AB5535 1:1000 7 Iba1 Goat Abcam AB5076 1:500 8 CD68 Rat Biolegend 137,001 1:500 10981136, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/glia.70013 by IN A SP - N E PA L , W iley O nline L ibrary on [07/04/2025].

Techniques: Control